Service Descriptions

Genome Editing Services

  1. gRNA Design and Production – Service will provide knockout, SNP knockin, exon floxing, tagging and domain deletion alleles.
  2. Pronuclear and cytoplasmic zygote injections – Service will provide mice with verified genome edited allele.

Gene Targeted Mouse Services

  1. Vector Evaluation (Gene Targeting) – Service will provide scientific review of a gene targeting vector.
  2. Vector Design and Construction / Simple Knockout (Gene Targeting) – With information provided by the researcher / investigator, GEMM Core will design and build a targeted vector.
  3. Vector Design and Construction / Conditional Knockout (Gene Targeting)- With information provided by the researcher / investigator, GEMM Core will design and build a targeted vector.
  4. Vector Design and Construction / Conditional Knockin (Gene Targeting)- With information provided by the researcher / investigator, GEMM Core will design and build a targeted vector.
  5. DNA Electroporation into Mouse ES Cells – Service will genetically modify a specific gene in mouse ES cells.
  6. Transient Recombinase Expression in ES Cells – Service provides electroporation of temporary gene into ES cells.
  7. Blastocyst Injection – Service will produce chimeric mice from blastocyst microinjections of genetically modified mouse embryonic stem cells.

Transgenic Mouse Services

  1. Vector Evaluation (Transgenic)- Service will provide scientific review of a transgenic vector.
  2. Vector Design and Construction / Simple (Transgenic) – With information provided by the researcher / investigator, GEMM Core will design and build a transgenic vector.
  3. Vector Design and Construction / Conditional (Transgenic) – With information provided by the researcher / investigator, GEMM Core will design and build a transgenic vector.
  4. Pronuclear Injection - Service will produce transgenic mice through the microinjection of DNA sequences into fertilized mouse zygotes.

Embryo Rederivation Services

  1. Embryo Rederivation – Service will eliminate detrimental mouse pathogens.

Liquid Nitrogen Storage

  1. Sperm Cyropreservation – Service will provide cryogenic archiving of mouse gametes.
  2. Annual LN2 Cyrogenic Storage Fee – Annual fee for storing cryogenically archived mouse sperm samples.

Other GEMM Core Services

  1. GEMM Core / labor – GEMM Core personnel's hourly rate. This rate is charged for any services not listed. Please contact GEMM Core for further details on this service.
  2. Grant Section Writing / Figure Preparation - GEMM Core personnel's hourly rate. Service provides vector design and figure preparation to assist with grant submissions.
  3. PCR Genotype Troubleshooting – GEMM Core personnel's hourly rate. Service provides troubleshooting and review of PCR genotyping techniques.
  4. Short Diagnostic PCR Reaction Development- GEMM Core personnel's hourly rate. Service provides design and testing of PCR reactions for screening mice cells and tissues.
  5. Non GEMM Core ES Cell Clone Expansion- Service provides expansion from one vial to 5 with cryogenic storage for up to 2 months.
  6. In Vitro Fertilization (mouse)- Service will re-establish a mouse line by In Vitro Fertilization using frozen or fresh sperm.